Properties

Related Categories

Angiogenesis, Angiogenic Factors, Cancer Research, Cell Biology, Cell Culture.

Biological source

Human

Recombinant

Expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

Form

Lyophilized

Molecular weight

8.8 kDa

Packaging

20 μg pack

General description

Prokinethicin-2 (PK2) is a secreted cysteine-rich protein that is expressed in the testes and lower levels of the small intestine. PK2 regulates several biological functions including gastrointestinal motility, angiogenesis, and circadian rhythms. It is closely related to EG-VEGF (Prokineticin-1) and binds to two orphan B protein-coupled receptors called PK-R1 and PK-R2.

Recombinant human procyanidin-2 is an 8.8 kDa protein consisting of 81 amino acid residues and ten cysteine ​​residues that potentially form five intramolecular disulfide bonds. It also contains a fully conserved N-terminal hexapeptide sequence that participates in biological activity. PK2 is encoded by the gene mapped to human chromosome 3p13.

Biochemical / Physiological Actions

Prokinethicin-2 (PK2) plays a vital role in the regulation of various biological functions, such as gastrointestinal motility, angiogenesis, and circadian rhythms. It is closely related to EG-VEGF (endocrine gland-derived vascular endothelial growth factor) / prokinethicin-1 (PK1). PK1 and PK2 exert their function by interacting with two G protein-coupled receptors PKR1 and PKR2, respectively. PK2 is a candidate gene for the pathogenesis of mood disorders.

Physical form

Freeze-dried without additives.

Reconstitution

Centrifuge the vial before opening. Reconstitute in water at a concentration of 0.1-1.0 mg / ml. Do not shake. This solution can be stored at 2-8 ° C for up to 1 week. For long-term storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g. 0.1% BSA) and store in working aliquots at -20 ° C to -80 ° C.