Degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) via the method. The higher temperature is very useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this can end in non-enzymatic RNA degradation.

DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the look at of lariat RNA produced by typical splicing, along with circRNAs which come up via back-splicing. By eradicating linear
RNAs from cellular or RNA extracts, RNase R drastically facilitates the identification of spherical species via RNA-sequencing. This allows researchers to probe the panorama of splicing events with greater depth.

APPLICATIONS:

• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying numerous splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Avoid repeated freeze-thaw cycles of all elements to retain most effectivity. All elements
are regular for one 12 months from the date of transport when saved and handled accurately.

 

Cat # +Dimension	M1228-500
Dimension	500 U
Highlights	RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions	-20°C
Supply Conditions	Gel Pack
USAGE	For Evaluation Use Solely! Not For Use in Individuals.

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are typically required.

• RNase R shows low train on tRNA, rRNA and completely different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use further RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Do not forget that spherical RNAs symbolize a small proportion of full RNA (typically 0.1%-0.01%), as a result of this truth RNase R treatment will most definitely finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is helpful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be helpful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation may be utilized as an alternative. For NGS, sturdy half reversible immobilization (SPRI) bead cleanup is helpful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

RELATED PRODUCTS:

• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

 

RNase H (RNase-free)
K1093-500	ApexBio	500U	EUR 24
Description: RNase H

RNase H (RNase-free)
K1093-5000	ApexBio	5000U	EUR 120
Description: RNase H

RNAse
E16FXP0211R-200	EnoGene	200 μl	EUR 13.33
Description: Available in various conjugation types.

RNAse
E16FXP0211R-400	EnoGene	400 μl	EUR 20
Description: Available in various conjugation types.

RNAse
E16FXP0211R-800	EnoGene	800 μl	EUR 26.67
Description: Available in various conjugation types.

RNase A
G117	ABM	25 mg	EUR 55
Description: RNase A is pancreatic bovine endoribonuclease that cleaves single stranded RNA by hydrolysis at cytosine and uracil residues. RNase A is very useful for the removal of RNA from plasmid DNA preparations.

PI / RNAse
abx290029-200tests	Abbexa	200 tests	EUR 326.4

RNase A
abx098139-1ml	Abbexa	1 ml	EUR 260.4

RNase A
M1227-25	Biovision	each	EUR 294

RNase H
RK20535	Abclonal	250U	EUR 88

RNase A
RN103-001	GenDepot	300mg	EUR 3138

RNase A
RB0473	Bio Basic	500mg	EUR 219.73

RNase A
IB47163	IBI Scientific	50ul	EUR 20.72
Description: (FOR HI-SPEED PLASMID) [FR}

RNase A
MB087-100MG	EWC Diagnostics	1 unit	EUR 34.02
Description: RNase A

RNase A
MB087-1G	EWC Diagnostics	1 unit	EUR 284.22
Description: RNase A

RNase A
MB087-500MG	EWC Diagnostics	1 unit	EUR 153.8
Description: RNase A

RNase A
RM1555-100MG	EWC Diagnostics	1 unit	EUR 118.89
Description: RNase A

RNase A
RM1555-500MG	EWC Diagnostics	1 unit	EUR 537.26
Description: RNase A

RNase A
RM7705-100MG	EWC Diagnostics	1 unit	EUR 136.11
Description: RNase A