RNAs from cellular or RNA extracts

Degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) via the method. The higher temperature is very useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this can end in non-enzymatic RNA degradation.

DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the look at of lariat RNA produced by typical splicing, along with circRNAs which come up via back-splicing. By eradicating linear
RNAs from cellular or RNA extracts, RNase R drastically facilitates the identification of spherical species via RNA-sequencing. This allows researchers to probe the panorama of splicing events with greater depth.

APPLICATIONS:

• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying numerous splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Avoid repeated freeze-thaw cycles of all elements to retain most effectivity. All elements
are regular for one 12 months from the date of transport when saved and handled accurately.

 

Biovision rnase-r
Biovision rnase-r
Cat # +Dimension M1228-500
Dimension 500 U
Highlights RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions -20°C
Supply Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are typically required.

• RNase R shows low train on tRNA, rRNA and completely different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use further RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Do not forget that spherical RNAs symbolize a small proportion of full RNA (typically 0.1%-0.01%), as a result of this truth RNase R treatment will most definitely finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is helpful for a lot of downstream features.

• Whereas the enzyme could also be heat inactivated the method should not be helpful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation may be utilized as an alternative. For NGS, sturdy half reversible immobilization (SPRI) bead cleanup is helpful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

RELATED PRODUCTS:

• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

 

Fungi/ Yeast Genomic DNA Extraction Mini Kit(50prep)- RNase A
FAFYG001-RNASE 50 preps
EUR 303
Fungi/ Yeast Genomic DNA Extraction Mini Kit(100prep)- RNase A
FAFYG001-1-RNASE 100 preps
EUR 448
RNase A
abx098139-1ml 1 ml
EUR 217
  • Shipped within 5-10 working days.
PI / RNAse
abx290029-200tests 200 tests
EUR 272
  • Shipped within 2-3 weeks.
RNase A
M1227-25
EUR 245
RNase A
RN103-001 300mg
EUR 2615
RNase A
RB0473 500mg
EUR 183.11
  • Product category: Proteins/Enzymes/RNase
RNAse H2A Antibody
24819-100ul 100ul
EUR 390
Rnase (90–105)
5-01885 4 x 1mg Ask for price
RNase A (MBG)
409-050 50 mg
EUR 73
RNase A (MBG)
409-250 250 mg
EUR 193
DNase (RNase-free)
abx098138-1500U 1500 U
EUR 453
  • Shipped within 5-10 working days.
RNase-Free water
abx098145-25ml 25 ml
EUR 175
  • Shipped within 5-10 working days.
RNase 1 Antibody
20-abx141499
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.
RNase 13 Antibody
20-abx141582
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.
Rnase A, Pancreatic
abx082443-25mg 25 mg
EUR 189
  • Shipped within 5-10 working days.
RiboSafe RNase Inhibitor
BIO-65027 2500 Units Ask for price
RiboSafe RNase Inhibitor
BIO-65028 10,000 Units Ask for price
RNase T2 Antibody
abx237324-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Leave a Reply

Your email address will not be published. Required fields are marked *